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Barton, L.J., Wilmington, S.R., Martin, M.J., Skopec, H.M., Lovander, K.E., Pinto, B.S., Geyer, P.K. (2014). Unique and Shared Functions of Nuclear Lamina LEM Domain Proteins in Drosophila.  Genetics 197(2): 653--665.
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Research paper

The nuclear lamina is an extensive protein network that contributes to nuclear structure and function. LEM domain (LAP2, emerin, MAN1 domain, LEM-D) proteins are components of the nuclear lamina, identified by a shared ∼45-amino-acid motif that binds Barrier-to-autointegration factor (BAF), a chromatin-interacting protein. Drosophila melanogaster has three nuclear lamina LEM-D proteins, named Otefin (Ote), Bocksbeutel (Bocks), and dMAN1. Although these LEM-D proteins are globally expressed, loss of either Ote or dMAN1 causes tissue-specific defects in adult flies that differ from each other. The reason for such distinct tissue-restricted defects is unknown. Here, we generated null alleles of bocks, finding that loss of Bocks causes no overt adult phenotypes. Next, we defined phenotypes associated with lem-d double mutants. Although the absence of individual LEM-D proteins does not affect viability, loss of any two proteins causes lethality. Mutant phenotypes displayed by lem-d double mutants differ from baf mutants, suggesting that BAF function is retained in animals with a single nuclear lamina LEM-D protein. Interestingly, lem-d double mutants displayed distinct developmental and cellular mutant phenotypes, suggesting that Drosophila LEM-D proteins have developmental functions that are differentially shared with other LEM-D family members. This conclusion is supported by studies showing that ectopically produced LEM-D proteins have distinct capacities to rescue the tissue-specific phenotypes found in single lem-d mutants. Our findings predict that cell-specific mutant phenotypes caused by loss of LEM-D proteins reflect both the constellation of LEM-D proteins within the nuclear lamina and the capacity of functional compensation of the remaining LEM-D proteins.

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PubMed Central ID
PMC4063922 (PMC) (EuropePMC)
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Personal communication to FlyBase

Location data for bocks deletion alleles.
Barton and Geyer, 2015.5.11, Location data for bocks deletion alleles. [FBrf0228389]

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    Aberrations (1)
    Alleles (14)
    Genes (8)
    Natural transposons (1)
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    Transgenic Constructs (4)