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Citation
Saccone, G., Louis, C., Zhang, H., Petrella, V., Natale, M., Perri, M., Salvemini, M. (2014). Male-specific phosphorylated SR proteins in adult flies of the Mediterranean Fruitfly Ceratitis capitata.  BMC Genet. 15 Suppl 2(): S6.
FlyBase ID
FBrf0226878
Publication Type
Research paper
Abstract

Alternative splicing is a widely used mechanism of gene regulation in sex determination pathways of Insects. In species from orders as distant as Diptera, Hymenoptera and Coleoptera, female differentiation relies on the activities of conserved splicing regulators, TRA and TRA-2, promoting female-specific expression of the global effector doublesex (dsx). Less understood is to what extent post-translational modifications of splicing regulators plays a role in this pathway. In Drosophila melanogaster phosphorylation of TRA, TRA-2 and the general RBP1 factor by the LAMMER kinase doa (darkener of apricot) is required for proper female sex determination. To explore whether this is a general feature of the pathway we examined sex-specific differences in phosphorylation levels of SR splicing factors in the dipteran species D. melanogaster, Ceratitis capitata (Medfly) and Musca domestica (Housefly). We found a distinct and reproducible pattern of male-specific phosphorylation on protein extracts enriched for SR proteins in C. capitata suggesting that differential phosphorylation may also contribute to the regulation of sex-specific splicing in the Medfly.

PubMed ID
PubMed Central ID
PMC4255826 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    BMC Genet.
    Title
    BMC Genetics
    Publication Year
    2000-
    ISBN/ISSN
    1471-2156
    Data From Reference
    Genes (12)