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Citation
Vandersmissen, H.P., Van Hiel, M.B., Van Loy, T., Vleugels, R., Vanden Broeck, J. (2014). Silencing D. melanogaster lgr1 impairs transition from larval to pupal stage.  Gen. Comp. Endocrinol. 209(): 135--147.
FlyBase ID
FBrf0226918
Publication Type
Research paper
Abstract

G protein-coupled receptors (GPCRs) play key roles in a wide diversity of physiological processes and signalling pathways. The leucine-rich repeats containing GPCRs (LGRs) are a subfamily that is well-conserved throughout most metazoan phyla and have important regulatory roles in vertebrates. Here, we report on the critical role of Drosophila melanogaster LGR1, the fruit fly homologue of the vertebrate glycoprotein hormone receptors, in development as a factor involved in the regulation of pupariation. Transcript profiling revealed that lgr1 transcripts are most abundant in third instar larvae and adult flies. The tissues displaying the highest transcript levels were the hindgut, the rectum and the salivary glands. Knockdown using RNA interference (RNAi) demonstrated that white pupa formation was severely suppressed in D. melanogaster lgr1 RNAi larvae. Associated with this developmental defect was a reduced ecdysteroid titer, which is in line with significantly reduced transcript levels detected for the Halloween genes shadow (sad) and spookier (spok) in the third instar lgr1 RNAi larvae compared to the control condition.

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Gen. Comp. Endocrinol.
    Title
    General and Comparative Endocrinology
    Publication Year
    1961-
    ISBN/ISSN
    0016-6480
    Data From Reference
    Genes (7)