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Citation
Pathak, R.U., Srinivasan, A., Mishra, R.K. (2014). Genome-wide mapping of matrix attachment regions in Drosophila melanogaster.  BMC Genomics 15(): 1022.
FlyBase ID
FBrf0227035
Publication Type
Research paper
Abstract

Eukaryotic genome acquires functionality upon proper packaging within the nucleus. This process is facilitated by the structural framework of Nuclear Matrix, a nucleo-proteinaceous meshwork. Matrix Attachment Regions (MARs) in the genome serve as anchoring sites to this framework. Here we report direct sequencing of the MAR preparation from Drosophila melanogaster embryos and identify >7350 MARs. This amounts to ~2.5% of the fly genome and often coincide with AT rich non-coding regions. We find significant association of MARs with the origins of replication, transcription start sites, paused RNA Polymerase II sites and exons, but not introns, of highly expressed genes. We also identified sequence motifs and repeats that constitute MARs. Our data reveal the contact points of genome to the nuclear architecture and provide a link between nuclear functions and genomic packaging.

PubMed ID
PubMed Central ID
PMC4301625 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    BMC Genomics
    Title
    BMC Genomics
    Publication Year
    2001-
    ISBN/ISSN
    1471-2164
    Data From Reference