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Citation
Blackney, M.J., Cox, R., Shepherd, D., Parker, J.D. (2014). Cloning and expression analysis of Drosophila extracellular Cu Zn superoxide dismutase.  Biosci. Rep. 34(6): e00164.
FlyBase ID
FBrf0227049
Publication Type
Research paper
Abstract

In the present study, we cloned and sequenced the mRNAs of the Sod3 <up>extracellular Cu Zn SOD (superoxide dismutase)</up> gene in Drosophila and identified two mRNA products formed by alternative splicing. These products code for a long and short protein derived from the four transcripts found in global expression studies (Flybase numbers Dmel\CG9027, FBgn0033631). Both mRNA process variants contain an extracellular signalling sequence, a region of high homology to the Sod1 (cytoplasmic Cu Zn SOD) including a conserved AUG start, with the longer form also containing a hydrophobic tail. The two fully processed transcripts are homologous to Caenorhabditis elegans Sod3 mRNA showing the same processing pattern. Using an established KG p-element+ insertion line (KG06029), we demonstrate that the Sod3 codes for an active Cu Zn SOD. We found differing expression patterns across sex with higher levels of expression of Sod3 in females. There is a correlation of Sod1 and Sod3 gene expression and activity that can explain why Sod3 was not seen in earlier studies of Sod1. Finally, we found no effect on lifespan with the Sod3 hypomorph mutation (Sod3KG06029) but did observe a significant increase in resistance to paraquat and H2O2 (hydrogen peroxide).

PubMed ID
PubMed Central ID
PMC4274663 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Biosci. Rep.
    Title
    Bioscience Reports
    Publication Year
    1981-
    ISBN/ISSN
    0144-8463
    Data From Reference
    Alleles (6)
    Gene Groups (1)
    Genes (3)
    Insertions (1)