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Chen, Y.W., Song, S., Weng, R., Verma, P., Kugler, J.M., Buescher, M., Rouam, S., Cohen, S.M. (2014). Systematic Study of Drosophila MicroRNA Functions Using a Collection of Targeted Knockout Mutations.  Dev. Cell 31(6): 784--800.
FlyBase ID
FBrf0227064
Publication Type
Research paper
Abstract

MicroRNAs are abundant in animal genomes, yet little is known about their functions in vivo. Here, we report the production of 80 new Drosophila miRNA mutants by targeted homologous recombination. These mutants remove 104 miRNAs. Together with 15 previously reported mutants, this collection includes 95 mutants deleting 130 miRNAs. Collectively, these genes produce over 99% of all Drosophila miRNAs, measured by miRNA sequence reads. We present a survey of developmental and adult miRNA phenotypes. Over 80% of the mutants showed at least one phenotype using a p < 0.01 significance threshold. We observed a significant correlation between miRNA abundance and phenotypes related to survival and lifespan, but not to most other phenotypes. miRNA cluster mutants were no more likely than single miRNA mutants to produce significant phenotypes. This mutant collection will provide a resource for future analysis of the biological roles of Drosophila miRNAs.

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Dev. Cell
    Title
    Developmental Cell
    Publication Year
    2001-
    ISBN/ISSN
    1534-5807 1878-1551
    Data From Reference
    Aberrations (71)
    Alleles (79)
    Genes (174)
    Insertions (79)
    Experimental Tools (2)
    Transgenic Constructs (3)