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Citation
Bell, G.P., Fletcher, G.C., Brain, R., Thompson, B.J. (2015). Aurora kinases phosphorylate lgl to induce mitotic spindle orientation in Drosophila epithelia.  Curr. Biol. 25(1): 61--68.
FlyBase ID
FBrf0227227
Publication Type
Research paper
Abstract

The Lethal giant larvae (Lgl) protein was discovered in Drosophila as a tumor suppressor in both neural stem cells (neuroblasts) and epithelia. In neuroblasts, Lgl relocalizes to the cytoplasm at mitosis, an event attributed to phosphorylation by mitotically activated aPKC kinase and thought to promote asymmetric cell division. Here we show that Lgl also relocalizes to the cytoplasm at mitosis in epithelial cells, which divide symmetrically. The Aurora A and B kinases directly phosphorylate Lgl to promote its mitotic relocalization, whereas aPKC kinase activity is required only for polarization of Lgl. A form of Lgl that is a substrate for aPKC, but not Aurora kinases, can restore cell polarity in lgl mutants but reveals defects in mitotic spindle orientation in epithelia. We propose that removal of Lgl from the plasma membrane at mitosis allows Pins/LGN to bind Dlg and thus orient the spindle in the plane of the epithelium. Our findings suggest a revised model for Lgl regulation and function in both symmetric and asymmetric cell divisions.

PubMed ID
PubMed Central ID
PMC4291145 (PMC) (EuropePMC)
Related Publication(s)
Note

Epithelial cell division: aurora kicks lgl to the cytoplasmic curb.
Nakajima and Gibson, 2015, Curr. Biol. 25(1): R43--R45 [FBrf0227308]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Curr. Biol.
    Title
    Current Biology
    Publication Year
    1991-
    ISBN/ISSN
    0960-9822
    Data From Reference