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Citation
Eikenes, A.H., Malerød, L., Christensen, A.L., Steen, C.B., Mathieu, J., Nezis, I.P., Liestøl, K., Huynh, J.R., Stenmark, H., Haglund, K. (2015). ALIX and ESCRT-III Coordinately Control Cytokinetic Abscission during Germline Stem Cell Division In Vivo.  PLoS Genet. 11(1): e1004904.
FlyBase ID
FBrf0227451
Publication Type
Research paper
Abstract
Abscission is the final step of cytokinesis that involves the cleavage of the intercellular bridge connecting the two daughter cells. Recent studies have given novel insight into the spatiotemporal regulation and molecular mechanisms controlling abscission in cultured yeast and human cells. The mechanisms of abscission in living metazoan tissues are however not well understood. Here we show that ALIX and the ESCRT-III component Shrub are required for completion of abscission during Drosophila female germline stem cell (fGSC) division. Loss of ALIX or Shrub function in fGSCs leads to delayed abscission and the consequent formation of stem cysts in which chains of daughter cells remain interconnected to the fGSC via midbody rings and fusome. We demonstrate that ALIX and Shrub interact and that they co-localize at midbody rings and midbodies during cytokinetic abscission in fGSCs. Mechanistically, we show that the direct interaction between ALIX and Shrub is required to ensure cytokinesis completion with normal kinetics in fGSCs. We conclude that ALIX and ESCRT-III coordinately control abscission in Drosophila fGSCs and that their complex formation is required for accurate abscission timing in GSCs in vivo.
PubMed ID
PubMed Central ID
PMC4312039 (PMC) (EuropePMC)
Related Publication(s)
Personal communication to FlyBase
Location data for ALiX[3] deletion.
Haglund, 2015.5.18, Location data for ALiX[3] deletion. [FBrf0228442]
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    PLoS Genet.
    Title
    PLoS Genetics
    Publication Year
    2005-
    ISBN/ISSN
    1553-7404 1553-7390
    Data From Reference
    Alleles (15)
    Genes (3)
    Physical Interactions (2)
    Cell Lines (1)
    Natural transposons (2)
    Insertions (2)
    Experimental Tools (2)
    Transgenic Constructs (8)