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Citation
Diao, F., Ironfield, H., Luan, H., Diao, F., Shropshire, W.C., Ewer, J., Marr, E., Potter, C.J., Landgraf, M., White, B.H. (2015). Plug-and-Play Genetic Access to Drosophila Cell Types using Exchangeable Exon Cassettes.  Cell Rep. 10(8): 1410--1421.
FlyBase ID
FBrf0227693
Publication Type
Research paper
Abstract

Genetically encoded effectors are important tools for probing cellular function in living animals, but improved methods for directing their expression to specific cell types are required. Here, we introduce a simple, versatile method for achieving cell-type-specific expression of transgenes that leverages the untapped potential of "coding introns" (i.e., introns between coding exons). Our method couples the expression of a transgene to that of a native gene expressed in the cells of interest using intronically inserted "plug-and-play" cassettes (called "Trojan exons") that carry a splice acceptor site followed by the coding sequences of T2A peptide and an effector transgene. We demonstrate the efficacy of this approach in Drosophila using lines containing suitable MiMIC (Minos-mediated integration cassette) transposons and a palette of Trojan exons capable of expressing a range of commonly used transcription factors. We also introduce an exchangeable, MiMIC-like Trojan exon construct that can be targeted to coding introns using the Crispr/Cas system.

Graphical Abstract
Obtained with permission from Cell Press.
PubMed ID
PubMed Central ID
PMC4373654 (PMC) (EuropePMC)
Related Publication(s)
Personal communication to FlyBase

Transgenic donor lines containing Trojan cassettes.
White, 2015.5.28, Transgenic donor lines containing Trojan cassettes. [FBrf0228445]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Cell Rep.
    Title
    Cell reports
    ISBN/ISSN
    2211-1247
    Data From Reference
    Alleles (91)
    Genes (25)
    Molecular Constructs (31)
    Natural transposons (2)
    Insertions (58)
    Experimental Tools (15)
    Transgenic Constructs (39)
    Transcripts (9)