Open Close
Reference
Citation
Tritto, P., Palumbo, V., Micale, L., Marzulli, M., Bozzetti, M.P., Specchia, V., Palumbo, G., Pimpinelli, S., Berloco, M. (2015). Loss of Pol32 in Drosophila melanogaster Causes Chromosome Instability and Suppresses Variegation.  PLoS ONE 10(3): e0120859.
FlyBase ID
FBrf0228002
Publication Type
Research paper
Abstract

Pol32 is an accessory subunit of the replicative DNA Polymerase δ and of the translesion Polymerase ζ. Pol32 is involved in DNA replication, recombination and repair. Pol32's participation in high- and low-fidelity processes, together with the phenotypes arising from its disruption, imply multiple roles for this subunit within eukaryotic cells, not all of which have been fully elucidated. Using pol32 null mutants and two partial loss-of-function alleles pol32rd1 and pol32rds in Drosophila melanogaster, we show that Pol32 plays an essential role in promoting genome stability. Pol32 is essential to ensure DNA replication in early embryogenesis and it participates in the repair of mitotic chromosome breakage. In addition we found that pol32 mutantssuppress position effect variegation, suggesting a role for Pol32 in chromatin architecture.

PubMed ID
PubMed Central ID
PMC4380491 (PMC) (EuropePMC)
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    PLoS ONE
    Title
    PLoS ONE
    Publication Year
    2006-
    ISBN/ISSN
    1932-6203
    Data From Reference
    Aberrations (2)
    Alleles (6)
    Genes (5)
    Natural transposons (1)
    Insertions (4)
    Transgenic Constructs (2)