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Citation
Nagarkar-Jaiswal, S., Lee, P.T., Campbell, M.E., Chen, K., Anguiano-Zarate, S., Cantu Gutierrez, M., Busby, T., Lin, W.W., He, Y., Schulze, K.L., Booth, B.W., Evans-Holm, M., Venken, K.J., Levis, R.W., Spradling, A.C., Hoskins, R.A., Bellen, H.J. (2015). A library of MiMICs allows tagging of genes and reversible, spatial and temporal knockdown of proteins in Drosophila.  eLife 4(): e05338.
FlyBase ID
FBrf0228008
Publication Type
Research paper
Abstract
Here, we document a collection of ∼7434 MiMIC (Minos Mediated Integration Cassette) insertions of which 2854 are inserted in coding introns. They allowed us to create a library of 400 GFP-tagged genes. We show that 72% of internally tagged proteins are functional, and that more than 90% can be imaged in unfixed tissues. Moreover, the tagged mRNAs can be knocked down by RNAi against GFP (iGFPi), and the tagged proteins can be efficiently knocked down by deGradFP technology. The phenotypes associated with RNA and protein knockdown typically correspond to severe loss of function or null mutant phenotypes. Finally, we demonstrate reversible, spatial, and temporal knockdown of tagged proteins in larvae and adult flies. This new strategy and collection of strains allows unprecedented in vivo manipulations in flies for many genes. These strategies will likely extend to vertebrates.
PubMed ID
PubMed Central ID
PMC4379497 (PMC) (EuropePMC)
Related Publication(s)
Personal communication to FlyBase
Data submitted by the Gene Disruption Project to FlyBase for the RMCE-MiMIC PT-GFSTF insertion line collection.
Gene Disruption Project members, 2015-, Data submitted by the Gene Disruption Project to FlyBase for the RMCE-MiMIC PT-GFSTF insertion line collection. [FBrf0229138]
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    eLife
    Title
    eLife
    ISBN/ISSN
    2050-084X
    Data From Reference
    Alleles (310)
    List limited to the first 200 records. Use the HitList export button in the left sidebar to view all records.
    Genes (200)
    Datasets (2)
    Insertions (200)
    Transgenic Constructs (12)