Reference
Citation
Gao, X.J., Riabinina, O., Li, J., Potter, C.J., Clandinin, T.R., Luo, L. (2015). A transcriptional reporter of intracellular Ca(2+) in Drosophila.  Nat. Neurosci. 18(6): 917--925.
FlyBase ID
FBrf0228535
Publication Type
Research paper
Abstract
Intracellular Ca(2+) is a widely used neuronal activity indicator. Here we describe a transcriptional reporter of intracellular Ca(2+) (TRIC) in Drosophila that uses a binary expression system to report Ca(2+)-dependent interactions between calmodulin and its target peptide. We found that in vitro assays predicted in vivo properties of TRIC and that TRIC signals in sensory systems depend on neuronal activity. TRIC was able to quantitatively monitor neuronal responses that changed slowly, such as those of neuropeptide F-expressing neurons to sexual deprivation and neuroendocrine pars intercerebralis cells to food and arousal. Furthermore, TRIC-induced expression of a neuronal silencer in nutrient-activated cells enhanced stress resistance, providing a proof of principle that TRIC can be used for circuit manipulation. Thus, TRIC facilitates the monitoring and manipulation of neuronal activity, especially those reflecting slow changes in physiological states that are poorly captured by existing methods. TRIC's modular design should enable optimization and adaptation to other organisms.
PubMed ID
PubMed Central ID
PMC4446202 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Nat. Neurosci.
    Title
    Nature Neuroscience
    Publication Year
    1998-
    ISBN/ISSN
    1097-6256
    Data From Reference
    Alleles (22)
    Genes (10)
    Cell Lines (1)
    Natural transposons (3)
    Insertions (15)
    Experimental Tools (9)
    Transgenic Constructs (21)