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Citation
Yamamoto-Hino, M., Yoshida, H., Ichimiya, T., Sakamura, S., Maeda, M., Kimura, Y., Sasaki, N., Aoki-Kinoshita, K.F., Kinoshita-Toyoda, A., Toyoda, H., Ueda, R., Nishihara, S., Goto, S. (2015). Phenotype-based clustering of glycosylation-related genes by RNAi-mediated gene silencing.  Genes Cells 20(6): 521--542.
FlyBase ID
FBrf0228609
Publication Type
Research paper
Abstract

Glycan structures are synthesized by a series of reactions conducted by glycosylation-related (GR) proteins such as glycosyltransferases, glycan-modifying enzymes, and nucleotide-sugar transporters. For example, the common core region of glycosaminoglycans (GAGs) is sequentially synthesized by peptide-O-xylosyltransferase, β1,4-galactosyltransferase I, β1,3-galactosyltransferase II, and β1,3-glucuronyltransferase. This raises the possibility that functional impairment of GR proteins involved in synthesis of the same glycan might result in the same phenotypic abnormality. To examine this possibility, comprehensive silencing of genes encoding GR and proteoglycan core proteins was conducted in Drosophila. Drosophila GR candidate genes (125) were classified into five functional groups for synthesis of GAGs, N-linked, O-linked, Notch-related, and unknown glycans. Spatiotemporally regulated silencing caused a range of malformed phenotypes that fell into three types: extra veins, thick veins, and depigmentation. The clustered phenotypes reflected the biosynthetic pathways of GAGs, Fringe-dependent glycan on Notch, and glycans placed at or near nonreducing ends (herein termed terminal domains of glycans). Based on the phenotypic clustering, CG33145 was predicted to be involved in formation of terminal domains. Our further analysis showed that CG33145 exhibited galactosyltransferase activity in synthesis of terminal N-linked glycans. Phenotypic clustering, therefore, has potential for the functional prediction of novel GR genes.

PubMed ID
PubMed Central ID
PMC4682476 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Genes Cells
    Title
    Genes to cells : devoted to molecular & cellular mechanisms
    Publication Year
    1996-
    ISBN/ISSN
    1356-9597
    Data From Reference
    Alleles (87)
    Gene Groups (8)
    Genes (126)
    Natural transposons (1)
    Insertions (7)
    Experimental Tools (1)
    Transgenic Constructs (79)