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Fulga, T.A., McNeill, E.M., Binari, R., Yelick, J., Blanche, A., Booker, M., Steinkraus, B.R., Schnall-Levin, M., Zhao, Y., DeLuca, T., Bejarano, F., Han, Z., Lai, E.C., Wall, D.P., Perrimon, N., Van Vactor, D. (2015). A transgenic resource for conditional competitive inhibition of conserved Drosophila microRNAs.  Nat. Commun. 6(): 7279.
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Publication Type
Research paper

Although the impact of microRNAs (miRNAs) in development and disease is well established, understanding the function of individual miRNAs remains challenging. Development of competitive inhibitor molecules such as miRNA sponges has allowed the community to address individual miRNA function in vivo. However, the application of these loss-of-function strategies has been limited. Here we offer a comprehensive library of 141 conditional miRNA sponges targeting well-conserved miRNAs in Drosophila. Ubiquitous miRNA sponge delivery and consequent systemic miRNA inhibition uncovers a relatively small number of miRNA families underlying viability and gross morphogenesis, with false discovery rates in the 4–8% range. In contrast, tissue-specific silencing of muscle-enriched miRNAs reveals a surprisingly large number of novel miRNA contributions to the maintenance of adult indirect flight muscle structure and function. A strong correlation between miRNA abundance and physiological relevance is not observed, underscoring the importance of unbiased screens when assessing the contributions of miRNAs to complex biological processes.

PubMed ID
PubMed Central ID
PMC4471878 (PMC) (EuropePMC)
Related Publication(s)
Personal communication to FlyBase

Additional UAS-mir-sponge constructs and insertions.
Van Vactor, 2015.6.15, Additional UAS-mir-sponge constructs and insertions. [FBrf0228774]

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Cook and Van Vactor, 2015.6.15, Special UAS-mir-sponge constructs from David Van Vector. [FBrf0228928]

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    Nat. Commun.
    Nature communications
    Data From Reference
    Alleles (155)
    Genes (148)
    Datasets (1)
    Natural transposons (1)
    Insertions (160)
    Experimental Tools (2)
    Transgenic Constructs (145)