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Reference
Citation
Nagarkar-Jaiswal, S., DeLuca, S.Z., Lee, P.T., Lin, W.W., Pan, H., Zuo, Z., Lv, J., Spradling, A.C., Bellen, H.J. (2015). A genetic toolkit for tagging intronic MiMIC containing genes.  eLife 4(): e08469.
FlyBase ID
FBrf0229067
Publication Type
Research paper
Abstract

Previously, we described a large collection of Minos-Mediated Integration Cassettes (MiMICs) that contain two phiC31 recombinase target sites and allow the generation of a new exon that encodes a protein tag when the MiMIC is inserted in a codon intron (Nagarkar-Jaiswal et al., 2015). These modified genes permit numerous applications including assessment of protein expression pattern, identification of protein interaction partners by immunoprecipitation followed by mass spec, and reversible removal of the tagged protein in any tissue. At present, these conversions remain time and labor-intensive as they require embryos to be injected with plasmid DNA containing the exon tag. In this study, we describe a simple and reliable genetic strategy to tag genes/proteins that contain MiMIC insertions using an integrated exon encoding GFP flanked by FRT sequences. We document the efficiency and tag 60 mostly uncharacterized genes.

PubMed ID
PubMed Central ID
PMC4499919 (PMC) (EuropePMC)
Related Publication(s)
Personal communication to FlyBase

Data submitted by the Gene Disruption Project to FlyBase for the RMCE-MiMIC PT-GFSTF insertion line collection.
Gene Disruption Project members, 2015-, Data submitted by the Gene Disruption Project to FlyBase for the RMCE-MiMIC PT-GFSTF insertion line collection. [FBrf0229138]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    eLife
    Title
    eLife
    ISBN/ISSN
    2050-084X
    Data From Reference
    Alleles (67)
    Genes (64)
    Datasets (1)
    Molecular Constructs (4)
    Polypeptides (10)
    Natural transposons (1)
    Insertions (66)
    Experimental Tools (7)
    Transgenic Constructs (3)