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Gene Disruption Project members, (2015-). Data submitted by the Gene Disruption Project to FlyBase for the RMCE-MiMIC PT-GFSTF insertion line collection. 
FlyBase ID
FBrf0229138
Publication Type
Personal communication to FlyBase
Abstract
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Text of Personal Communication
The RMCE-MiMIC PT-GFSTF collection (FBlc0000719) described in Nagarkar-Jaiswal et al 2015, eLife  4:e05338  (FBrf0228008) and Nagarkar-Jaiswal et al 2015, eLife  4:e08469  (FBrf0229067) consists of transgenic fly stocks in which the RMCE cassette in a progenitor Mi{MIC} insertion has been replaced with a protein trap cassette containing a EGFP-FlAsH-StrepII-TEV-3xFlag ("GFSTF") tag that is flanked on either side with a 4X(GlyGlySer) flexible linker.  A PCR assay was used to identify  RMCE events that inserted the cassette in the correct orientation to result in a fusion protein containing the internal tag.
The 'Associated Files' for this personal communication consist of .xlsx files that that were submitted to FlyBase at the time that each set of the fly stocks was sent to the Bloomington Drosophila Stock Center.  The .xlsx files contain data primarily concerning the progenitor MiMIC line used, the phase of the donor construct used in the RMCE event, and the identities of any gene(s) in which the RMCE-MiMIC element is inserted.  The gene associations are based on the FlyBase gene annotations at the time of submission of the data.  The column "Trap? Y/N" for each gene indicates whether the protein trap cassette is predicted to result in tagging any of the annotated protein isoforms encoded by the gene.  Note that this is a prediction and does not necessarily indicate whether or not there is experimental evidence that the protein encoded by the gene is tagged.  A "Y" (Yes) in this column does not indicate that the insertion tags all predictive protein isoforms of the gene with multiple annotated isoforms.
DOI
Related Publication(s)
Research paper
A library of MiMICs allows tagging of genes and reversible, spatial and temporal knockdown of proteins in Drosophila.
Nagarkar-Jaiswal et al., 2015, eLife 4: e05338 [FBrf0228008]
A genetic toolkit for tagging intronic MiMIC containing genes.
Nagarkar-Jaiswal et al., 2015, eLife 4: e08469 [FBrf0229067]
Associated Information
Comments
Associated Files
File date: 2015.7.23 ; File size: 130852 ; File format: xlsx ; File name: FlyBase_data_RMCE_batch1-4.xlsx
File date: 13.8.2015 ; File size: 19167 ; File format: xlsx ; File name: FlyBase_data_RMCE_batch5.xlsx
File date: 2016.3.14 ; File size: 15437 ; File format: xlsx ; File name: FlyBase_data_RMCE_batch6.xlsx
File date: 2016.6.15 ; File size: 15682 ; File format: xlsx ; File name: FlyBase_data_RMCE_batch7.xlsx
File date: 2016.8.30 ; File size: 17134 ; File format: xlsx ; File name: FB_BDSC_data_RMCE_batch8.xlsx
File date: 2016.12.6 ; File size: 15615 ; File format: xlsx ; File name: FB_BDSC_data_RMCE_batch9.GFSTF.xlsx
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    Language of Publication
    English
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    Data From Reference
    Alleles (666)
    List limited to the first 200 records. Use the HitList export button in the left sidebar to view all records.
    Genes (639)
    List limited to the first 200 records. Use the HitList export button in the left sidebar to view all records.
    Datasets (1)
    Natural transposons (1)
    Insertions (1213)
    List limited to the first 200 records. Use the HitList export button in the left sidebar to view all records.
    Transgenic Constructs (3)