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Citation
Bivik, C., Bahrampour, S., Ulvklo, C., Nilsson, P., Angel, A., Fransson, F., Lundin, E., Renhorn, J., Thor, S. (2015). Novel Genes Involved in Controlling Specification of Drosophila FMRFamide Neuropeptide Cells.  Genetics 200(4): 1229--1244.
FlyBase ID
FBrf0229273
Publication Type
Research paper
Abstract

The expression of neuropeptides is often extremely restricted in the nervous system, making them powerful markers for addressing cell specification . In the developing Drosophila ventral nerve cord, only six cells, the Ap4 neurons, of some 10,000 neurons, express the neuropeptide FMRFamide (FMRFa). Each Ap4/FMRFa neuron is the last-born cell generated by an identifiable and well-studied progenitor cell, neuroblast 5-6 (NB5-6T). The restricted expression of FMRFa and the wealth of information regarding its gene regulation and Ap4 neuron specification makes FMRFa a valuable readout for addressing many aspects of neural development, i.e., spatial and temporal patterning cues, cell cycle control, cell specification, axon transport, and retrograde signaling. To this end, we have conducted a forward genetic screen utilizing an Ap4-specific FMRFa-eGFP transgenic reporter as our readout. A total of 9781 EMS-mutated chromosomes were screened for perturbations in FMRFa-eGFP expression, and 611 mutants were identified. Seventy-nine of the strongest mutants were mapped down to the affected gene by deficiency mapping or whole-genome sequencing. We isolated novel alleles for previously known FMRFa regulators, confirming the validity of the screen. In addition, we identified novel essential genes, including several with previously undefined functions in neural development. Our identification of genes affecting most major steps required for successful terminal differentiation of Ap4 neurons provides a comprehensive view of the genetic flow controlling the generation of highly unique neuronal cell types in the developing nervous system.

PubMed ID
PubMed Central ID
PMC4574234 (PMC) (EuropePMC)
Related Publication(s)
Personal communication to FlyBase

EMS alleles from Stefan Thor.
Thor, 2015.2.19, EMS alleles from Stefan Thor. [FBrf0227640]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Genetics
    Title
    Genetics
    Publication Year
    1916-
    ISBN/ISSN
    0016-6731
    Data From Reference
    Aberrations (6)
    Alleles (136)
    Genes (59)
    Natural transposons (1)
    Experimental Tools (2)
    Transgenic Constructs (2)