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Citation
Brooks, A.N., Duff, M.O., May, G., Yang, L., Bolisetty, M., Landolin, J., Wan, K., Sandler, J., Booth, B.W., Celniker, S.E., Graveley, B.R., Brenner, S.E. (2015). Regulation of alternative splicing in Drosophila by 56 RNA binding proteins.  Genome Res. 25(11): 1771--1780.
FlyBase ID
FBrf0230027
Publication Type
Research paper
Abstract

Alternative splicing is regulated by RNA binding proteins (RBPs) that recognize pre-mRNA sequence elements and activate or repress adjacent exons. Here, we used RNA interference and RNA-seq to identify splicing events regulated by 56 Drosophila proteins, some previously unknown to regulate splicing. Nearly all proteins affected alternative first exons, suggesting that RBPs play important roles in first exon choice. Half of the splicing events were regulated by multiple proteins, demonstrating extensive combinatorial regulation. We observed that SR and hnRNP proteins tend to act coordinately with each other, not antagonistically. We also identified a cross-regulatory network where splicing regulators affected the splicing of pre-mRNAs encoding other splicing regulators. This large-scale study substantially enhances our understanding of recent models of splicing regulation and provides a resource of thousands of exons that are regulated by 56 diverse RBPs.

PubMed ID
PubMed Central ID
PMC4617972 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Genome Res.
    Title
    Genome Research
    Publication Year
    1995-
    ISBN/ISSN
    1088-9051
    Data From Reference