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Citation
Onyenwoke, R.U., Sexton, J.Z., Yan, F., Díaz, M.C., Forsberg, L.J., Major, M.B., Brenman, J.E. (2015). The mucolipidosis IV Ca2+ channel TRPML1 (MCOLN1) is regulated by the TOR kinase.  Biochem. J. 470(3): 331--342.
FlyBase ID
FBrf0230366
Publication Type
Research paper
Abstract

Autophagy is a complex pathway regulated by numerous signalling events that recycles macromolecules and may be perturbed in lysosomal storage disorders (LSDs). During autophagy, aberrant regulation of the lysosomal Ca(2+) efflux channel TRPML1 <up>transient receptor potential mucolipin 1 (MCOLN1)</up>, also known as MCOLN1, is solely responsible for the human LSD mucolipidosis type IV (MLIV); however, the exact mechanisms involved in the development of the pathology of this LSD are unknown. In the present study, we provide evidence that the target of rapamycin (TOR), a nutrient-sensitive protein kinase that negatively regulates autophagy, directly targets and inactivates the TRPML1 channel and thereby functional autophagy, through phosphorylation. Further, mutating these phosphorylation sites to unphosphorylatable residues proved to block TOR regulation of the TRPML1 channel. These findings suggest a mechanism for how TOR activity may regulate the TRPML1 channel.

PubMed ID
PubMed Central ID
PMC4613499 (PMC) (EuropePMC)
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Biochem. J.
    Title
    The Biochemical Journal
    Publication Year
    1906-
    ISBN/ISSN
    0264-6021
    Data From Reference