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Citation
Molla-Herman, A., Vall├ęs, A.M., Ganem-Elbaz, C., Antoniewski, C., Huynh, J.R. (2015). tRNA processing defects induce replication stress and Chk2-dependent disruption of piRNA transcription.  EMBO J. 34(24): 3009--3027.
FlyBase ID
FBrf0230483
Publication Type
Research paper
Abstract

RNase P is a conserved endonuclease that processes the 5' trailer of tRNA precursors. We have isolated mutations in Rpp30, a subunit of RNase P, and find that these induce complete sterility in Drosophila females. Here, we show that sterility is not due to a shortage of mature tRNAs, but that atrophied ovaries result from the activation of several DNA damage checkpoint proteins, including p53, Claspin, and Chk2. Indeed, we find that tRNA processing defects lead to increased replication stress and de-repression of transposable elements in mutant ovaries. We also report that transcription of major piRNA sources collapse in mutant germ cells and that this correlates with a decrease in heterochromatic H3K9me3 marks on the corresponding piRNA-producing loci. Our data thus link tRNA processing, DNA replication, and genome defense by small RNAs. This unexpected connection reveals constraints that could shape genome organization during evolution.

PubMed ID
PubMed Central ID
PMC4687792 (PMC) (EuropePMC)
Related Publication(s)
Note

Misprocessed tRNA response targets piRNA clusters.
Yamanaka and Siomi, 2015, EMBO J. 34(24): 2988--2989 [FBrf0232017]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    EMBO J.
    Title
    The EMBO Journal
    Publication Year
    1982-
    ISBN/ISSN
    0261-4189
    Data From Reference