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Citation
Cipressa, F., Morciano, P., Bosso, G., Mannini, L., Galati, A., Daniela Raffa, G., Cacchione, S., Musio, A., Cenci, G. (2016). A role for Separase in telomere protection.  Nat. Commun. 7(): 10405.
FlyBase ID
FBrf0230695
Publication Type
Research paper
Abstract

Drosophila telomeres are elongated by transposition of specialized retroelements rather than telomerase activity and are assembled independently of the sequence. Fly telomeres are protected by the terminin complex that localizes and functions exclusively at telomeres and by non-terminin proteins that do not serve telomere-specific functions. We show that mutations in the Drosophila Separase encoding gene Sse lead not only to endoreduplication but also telomeric fusions (TFs), suggesting a role for Sse in telomere capping. We demonstrate that Separase binds terminin proteins and HP1, and that it is enriched at telomeres. Furthermore, we show that loss of Sse strongly reduces HP1 levels, and that HP1 overexpression in Sse mutants suppresses TFs, suggesting that TFs are caused by a HP1 diminution. Finally, we find that siRNA-induced depletion of ESPL1, the Sse human orthologue, causes telomere dysfunction and HP1 level reduction in primary fibroblasts, highlighting a conserved role of Separase in telomere protection.

PubMed ID
PubMed Central ID
PMC4735636 (PMC) (EuropePMC)
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Nat. Commun.
    Title
    Nature communications
    ISBN/ISSN
    2041-1723
    Data From Reference
    Aberrations (1)
    Alleles (11)
    Genes (11)
    Physical Interactions (11)
    Cell Lines (1)
    Natural transposons (1)
    Insertions (1)
    Transgenic Constructs (7)