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Citation
Sarov, M., Barz, C., Jambor, H., Hein, M.Y., Schmied, C., Suchold, D., Stender, B., Janosch, S., Kj, V.V., Krishnan, R.T., Krishnamoorthy, A., Ferreira, I.R., Ejsmont, R.K., Finkl, K., Hasse, S., Kämpfer, P., Plewka, N., Vinis, E., Schloissnig, S., Knust, E., Hartenstein, V., Mann, M., Ramaswami, M., VijayRaghavan, K., Tomancak, P., Schnorrer, F. (2016). A genome-wide resource for the analysis of protein localisation in Drosophila.  eLife 5(): e12068.
FlyBase ID
FBrf0231269
Publication Type
Research paper
Abstract
The Drosophila genome contains >13000 protein-coding genes, the majority of which remain poorly investigated. Important reasons include the lack of antibodies or reporter constructs to visualise these proteins. Here, we present a genome-wide fosmid library of 10000 GFP-tagged clones, comprising tagged genes and most of their regulatory information. For 880 tagged proteins, we created transgenic lines, and for a total of 207 lines, we assessed protein expression and localisation in ovaries, embryos, pupae or adults by stainings and live imaging approaches. Importantly, we visualised many proteins at endogenous expression levels and found a large fraction of them localising to subcellular compartments. By applying genetic complementation tests, we estimate that about two-thirds of the tagged proteins are functional. Moreover, these tagged proteins enable interaction proteomics from developing pupae and adult flies. Taken together, this resource will boost systematic analysis of protein expression and localisation in various cellular and developmental contexts.
PubMed ID
PubMed Central ID
PMC4805545 (PMC) (EuropePMC)
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Note
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    eLife
    Title
    eLife
    ISBN/ISSN
    2050-084X
    Data From Reference
    Alleles (878)
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    Genes (827)
    Natural transposons (2)
    Insertions (881)
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    Experimental Tools (10)
    Transgenic Constructs (879)
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