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Citation
Kuleesha, Y., Puah, W.C., Wasser, M. (2016). A model of muscle atrophy based on live microscopy of muscle remodelling in Drosophila metamorphosis.  R. Soc. Open Sci. 3(2): 150517.
FlyBase ID
FBrf0231322
Publication Type
Research paper
Abstract

Genes controlling muscle size and survival play important roles in muscle wasting diseases. In Drosophila melanogaster metamorphosis, larval abdominal muscles undergo two developmental fates. While a doomed population is eliminated by cell death, another persistent group is remodelled and survives into adulthood. To identify and characterize genes involved in the development of remodelled muscles, we devised a workflow consisting of in vivo imaging, targeted gene perturbation and quantitative image analysis. We show that inhibition of TOR signalling and activation of autophagy promote developmental muscle atrophy in early, while TOR and yorkie activation are required for muscle growth in late pupation. We discovered changes in the localization of myonuclei during remodelling that involve anti-polar migration leading to central clustering followed by polar migration resulting in localization along the midline. We demonstrate that the Cathepsin L orthologue Cp1 is required for myonuclear clustering in mid, while autophagy contributes to central positioning of nuclei in late metamorphosis. In conclusion, studying muscle remodelling in metamorphosis can provide new insights into the cell biology of muscle wasting.

PubMed ID
PubMed Central ID
PMC4785973 (PMC) (EuropePMC)
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    R. Soc. Open Sci.
    Title
    Royal Society open science
    ISBN/ISSN
    2054-5703
    Data From Reference
    Alleles (120)
    Genes (98)
    Natural transposons (1)
    Experimental Tools (2)
    Transgenic Constructs (120)