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Citation
Nelson, J.O., Moore, K.A., Chapin, A., Hollien, J., Metzstein, M.M. (2016). Degradation of Gadd45 mRNA by nonsense-mediated decay is essential for viability.  eLife 5(): e12876.
FlyBase ID
FBrf0232230
Publication Type
Research paper
Abstract

The nonsense-mediated mRNA decay (NMD) pathway functions to degrade both abnormal and wild-type mRNAs. NMD is essential for viability in most organisms, but the molecular basis for this requirement is unknown. Here we show that a single, conserved NMD target, the mRNA coding for the stress response factor growth arrest and DNA-damage inducible 45 (GADD45) can account for lethality in Drosophila lacking core NMD genes. Moreover, depletion of Gadd45 in mammalian cells rescues the cell survival defects associated with NMD knockdown. Our findings demonstrate that degradation of Gadd45 mRNA is the essential NMD function and, surprisingly, that the surveillance of abnormal mRNAs by this pathway is not necessarily required for viability.

PubMed ID
PubMed Central ID
PMC4848089 (PMC) (EuropePMC)
Related Publication(s)
Note

How cells kill a "killer" messenger.
Saveanu and Jacquier, 2016, eLife 5: e16076 [FBrf0232265]

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    eLife
    Title
    eLife
    ISBN/ISSN
    2050-084X
    Data From Reference
    Aberrations (377)
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    Alleles (13)
    Genes (9)
    Natural transposons (1)
    Insertions (4)
    Experimental Tools (2)
    Transgenic Constructs (3)