FB2025_01 , released February 20, 2025
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Stuurman, N., Vale, R.D. (2016.4.27). Comments on S2 cell lines used, Stuurman. 
FlyBase ID
FBrf0232277
Publication Type
Personal communication to FlyBase
Abstract
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Text of Personal Communication 
The following is in response to a query from Dr. Lucy Cherbas on the identity of the cultured cell line used in a number of publications.
After asking the various primary authors, I have the following
Moutinho-Pereira, S., Stuurman, N., Afonso, O., Hornsveld, M., Aguiar, P., Goshima, G., Vale, R.D., Maiato, H. (2013). Genes involved in centrosome-independent mitotic spindle assembly in Drosophila S2 cells.  Proc. Natl. Acad. Sci. U.S.A. 110(49): 19808--19813.
Most likely S2U.
Uehara, R., Nozawa, R.S., Tomioka, A., Petry, S., Vale, R.D., Obuse, C., Goshima, G. (2009). The augmin complex plays a critical role in spindle microtubule generation for mitotic progression and cytokinesis in human cells.  Proc. Natl. Acad. Sci. U.S.A. 106(17): 6998--7003.
S2U.
Vale, R.D., Spudich, J.A., Griffis, E.R. (2009). Dynamics of myosin, microtubules, and Kinesin-6 at the cortex during cytokinesis in Drosophila S2 cells.  J. Cell Biol. 186(5): 727--738.
S2U.
Goshima, G., Mayer, M., Zhang, N., Stuurman, N., Vale, R.D. (2008). Augmin: a protein complex required for centrosome-independent microtubule generation within the spindle.  J. Cell Biol. 181(3): 421--429.
S2U.
Goshima, G., Nedelec, F., Vale, R.D. (2005). Mechanisms for focusing mitotic spindle poles by minus end-directed motor proteins.  J. Cell Biol. 171(2): 229--240.
S2U.
Goshima, G., Vale, R.D. (2005). Cell cycle-dependent dynamics and regulation of mitotic kinesins in Drosophila S2 cells.  Mol. Biol. Cell 16(8): 3896--3907.
S2U.
Goshima, G., Wollman, R., Stuurman, N., Scholey, J.M., Vale, R.D. (2005). Length control of the metaphase spindle.  Curr. Biol. 15(22): 1979--1988.
S2U, except for Fig. S5 which used S2I.
DOI
Related Publication(s)
Research paper

Genes involved in centrosome-independent mitotic spindle assembly in Drosophila S2 cells.
Moutinho-Pereira et al., 2013, Proc. Natl. Acad. Sci. U.S.A. 110(49): 19808--19813 [FBrf0223512]

The augmin complex plays a critical role in spindle microtubule generation for mitotic progression and cytokinesis in human cells.
Uehara et al., 2009, Proc. Natl. Acad. Sci. U.S.A. 106(17): 6998--7003 [FBrf0227582]

Dynamics of myosin, microtubules, and Kinesin-6 at the cortex during cytokinesis in Drosophila S2 cells.
Vale et al., 2009, J. Cell Biol. 186(5): 727--738 [FBrf0208859]

Augmin: a protein complex required for centrosome-independent microtubule generation within the spindle.
Goshima et al., 2008, J. Cell Biol. 181(3): 421--429 [FBrf0204750]

Mechanisms for focusing mitotic spindle poles by minus end-directed motor proteins.
Goshima et al., 2005, J. Cell Biol. 171(2): 229--240 [FBrf0190934]

Cell cycle-dependent dynamics and regulation of mitotic kinesins in Drosophila S2 cells.
Goshima and Vale, 2005, Mol. Biol. Cell 16(8): 3896--3907 [FBrf0188027]

Length control of the metaphase spindle.
Goshima et al., 2005, Curr. Biol. 15(22): 1979--1988 [FBrf0190017]

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    Cell Lines (1)