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Citation
Lőrincz, P., Lakatos, Z., Varga, Á., Maruzs, T., Simon-Vecsei, Z., Darula, Z., Benkő, P., Csordás, G., Lippai, M., Andó, I., Hegedűs, K., Medzihradszky, K.F., Takáts, S., Juhász, G. (2016). MiniCORVET is a Vps8-containing early endosomal tether in Drosophila.  eLife 5(): e14226.
FlyBase ID
FBrf0232837
Publication Type
Research paper
Abstract

Yeast studies identified two heterohexameric tethering complexes, which consist of 4 shared (Vps11, Vps16, Vps18 and Vps33) and 2 specific subunits: Vps3 and Vps8 (CORVET) versus Vps39 and Vps41 (HOPS). CORVET is an early and HOPS is a late endosomal tether. The function of HOPS is well known in animal cells, while CORVET is poorly characterized. Here we show that Drosophila Vps8 is highly expressed in hemocytes and nephrocytes, and localizes to early endosomes despite the lack of a clear Vps3 homolog. We find that Vps8 forms a complex and acts together with Vps16A, Dor/Vps18 and Car/Vps33A, and loss of any of these proteins leads to fragmentation of endosomes. Surprisingly, Vps11 deletion causes enlargement of endosomes, similar to loss of the HOPS-specific subunits Vps39 and Lt/Vps41. We thus identify a 4 subunit-containing miniCORVET complex as an unconventional early endosomal tether in Drosophila.

PubMed ID
PubMed Central ID
PMC4935465 (PMC) (EuropePMC)
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    eLife
    Title
    eLife
    ISBN/ISSN
    2050-084X
    Data From Reference