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Citation
Honjo, K., Mauthner, S.E., Wang, Y., Skene, J.H., Tracey, W.D. (2016). Nociceptor-Enriched Genes Required for Normal Thermal Nociception.  Cell Rep. 16(2): 295--303.
FlyBase ID
FBrf0232848
Publication Type
Research paper
Abstract

Here, we describe a targeted reverse genetic screen for thermal nociception genes in Drosophila larvae. Using laser capture microdissection and microarray analyses of nociceptive and non-nociceptive neurons, we identified 275 nociceptor-enriched genes. We then tested the function of the enriched genes with nociceptor-specific RNAi and thermal nociception assays. Tissue-specific RNAi targeted against 14 genes caused insensitive thermal nociception while targeting of 22 genes caused hypersensitive thermal nociception. Previously uncategorized genes were named for heat resistance (i.e., boilerman, fire dancer, oven mitt, trivet, thawb, and bunker gear) or heat sensitivity (firelighter, black match, eucalyptus, primacord, jet fuel, detonator, gasoline, smoke alarm, and jetboil). Insensitive nociception phenotypes were often associated with severely reduced branching of nociceptor neurites and hyperbranched dendrites were seen in two of the hypersensitive cases. Many genes that we identified are conserved in mammals.

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Obtained with permission from Cell Press.
PubMed ID
PubMed Central ID
PMC5333372 (PMC) (EuropePMC)
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Cell Rep.
    Title
    Cell reports
    ISBN/ISSN
    2211-1247
    Data From Reference
    Alleles (39)
    Genes (276)
    Natural transposons (1)
    Experimental Tools (1)
    Transgenic Constructs (39)