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Citation
Navarro-Costa, P., McCarthy, A., PrudĂȘncio, P., Greer, C., Guilgur, L.G., Becker, J.D., Secombe, J., Rangan, P., Martinho, R.G. (2016). Early programming of the oocyte epigenome temporally controls late prophase I transcription and chromatin remodelling.  Nat. Commun. 7(): 12331.
FlyBase ID
FBrf0233144
Publication Type
Research paper
Abstract

Oocytes are arrested for long periods of time in the prophase of the first meiotic division (prophase I). As chromosome condensation poses significant constraints to gene expression, the mechanisms regulating transcriptional activity in the prophase I-arrested oocyte are still not entirely understood. We hypothesized that gene expression during the prophase I arrest is primarily epigenetically regulated. Here we comprehensively define the Drosophila female germ line epigenome throughout oogenesis and show that the oocyte has a unique, dynamic and remarkably diversified epigenome characterized by the presence of both euchromatic and heterochromatic marks. We observed that the perturbation of the oocyte's epigenome in early oogenesis, through depletion of the dKDM5 histone demethylase, results in the temporal deregulation of meiotic transcription and affects female fertility. Taken together, our results indicate that the early programming of the oocyte epigenome primes meiotic chromatin for subsequent functions in late prophase I.

PubMed ID
PubMed Central ID
PMC4987523 (PMC) (EuropePMC)
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Nat. Commun.
    Title
    Nature communications
    ISBN/ISSN
    2041-1723
    Data From Reference
    Alleles (8)
    Genes (38)
    Natural transposons (1)
    Experimental Tools (1)
    Transgenic Constructs (6)