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Citation
Ji, Y., Thomas, C., Tulin, N., Lodhi, N., Boamah, E., Kolenko, V., Tulin, A.V. (2016). Charon Mediates Immune Deficiency-Driven PARP-1-Dependent Immune Responses in Drosophila.  J. Immunol. 197(6): 2382--2389.
FlyBase ID
FBrf0233348
Publication Type
Research paper
Abstract

Regulation of NF-κB nuclear translocation and stability is central to mounting an effective innate immune response. In this article, we describe a novel molecular mechanism controlling NF-κB-dependent innate immune response. We show that a previously unknown protein, termed as Charon, functions as a regulator of antibacterial and antifungal immune defense in Drosophila Charon is an ankyrin repeat-containing protein that mediates poly(ADP-ribose) polymerase-1 (PARP-1)-dependent transcriptional responses downstream of the innate immune pathway. Our results demonstrate that Charon interacts with the NF-κB ortholog Relish inside perinuclear particles and delivers active Relish to PARP-1-bearing promoters, thus triggering NF-κB/PARP-1-dependent transcription of antimicrobial peptides. Ablating the expression of Charon prevents Relish from targeting promoters of antimicrobial genes and effectively suppresses the innate immune transcriptional response. Taken together, these results implicate Charon as an essential mediator of PARP-1-dependent transcription in the innate immune pathway. Thus, to our knowledge, our results are the first to describe the molecular mechanism regulating translocation of the NF-κB subunit from cytoplasm to chromatin.

PubMed ID
PubMed Central ID
PMC5010969 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    J. Immunol.
    Title
    Journal of Immunology
    Publication Year
    1950-
    ISBN/ISSN
    0022-1767
    Data From Reference
    Alleles (10)
    Gene Groups (1)
    Genes (7)
    Physical Interactions (2)
    Natural transposons (1)
    Insertions (1)
    Experimental Tools (2)
    Transgenic Constructs (7)