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Citation
Kockel, L., Huq, L.M., Ayyar, A., Herold, E., MacAlpine, E., Logan, M., Savvides, C., Kim, G.E., Chen, J., Clark, T., Duong, T., Fazel-Rezai, V., Havey, D., Han, S., Jagadeesan, R., Kim, E.S., Lee, D., Lombardo, K., Piyale, I., Shi, H., Stahr, L., Tung, D., Tayvah, U., Wang, F., Wang, J.H., Xiao, S., Topper, S.M., Park, S., Rotondo, C., Rankin, A.E., Chisholm, T.W., Kim, S.K. (2016). A Drosophila LexA Enhancer-Trap Resource for Developmental Biology and Neuroendocrine Research.  G3 (Bethesda) 6(10): 3017--3026.
FlyBase ID
FBrf0233719
Publication Type
Research paper
Abstract
Novel binary gene expression tools like the LexA-LexAop system could powerfully enhance studies of metabolism, development, and neurobiology in Drosophila However, specific LexA drivers for neuroendocrine cells and many other developmentally relevant systems remain limited. In a unique high school biology course, we generated a LexA-based enhancer trap collection by transposon mobilization. The initial collection provides a source of novel LexA-based elements that permit targeted gene expression in the corpora cardiaca, cells central for metabolic homeostasis, and other neuroendocrine cell types. The collection further contains specific LexA drivers for stem cells and other enteric cells in the gut, and other developmentally relevant tissue types. We provide detailed analysis of nearly 100 new LexA lines, including molecular mapping of insertions, description of enhancer-driven reporter expression in larval tissues, and adult neuroendocrine cells, comparison with established enhancer trap collections and tissue specific RNAseq. Generation of this open-resource LexA collection facilitates neuroendocrine and developmental biology investigations, and shows how empowering secondary school science can achieve research and educational goals.
PubMed ID
PubMed Central ID
PMC5068927 (PMC) (EuropePMC)
Related Publication(s)
Personal communication to FlyBase
StanEx Stocks.
Kockel, 2016.11.29, StanEx Stocks. [FBrf0233981]
StanEx Stocks.
Kockel, 2016.12.8, StanEx Stocks. [FBrf0234156]
Associated Information
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Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    G3 (Bethesda)
    Title
    G3 : genes - genomes - genetics
    ISBN/ISSN
    2160-1836
    Data From Reference
    Alleles (164)
    Genes (95)
    Natural transposons (1)
    Insertions (96)
    Experimental Tools (3)
    Transgenic Constructs (1)