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Citation
L Black, K., Petruk, S., Fenstermaker, T.K., Hodgson, J.W., Caplan, J.L., Brock, H.W., Mazo, A. (2016). Chromatin proteins and RNA are associated with DNA during all phases of mitosis.  Cell Discov. 2(): 16038.
FlyBase ID
FBrf0233883
Publication Type
Research paper
Abstract
Mitosis brings about major changes to chromosome and nuclear structure. We used recently developed proximity ligation assay-based techniques to investigate the association with DNA of chromatin-associated proteins and RNAs in Drosophila embryos during mitosis. All groups of tested proteins, histone-modifying and chromatin-remodeling proteins and methylated histones remained in close proximity to DNA during all phases of mitosis. We also found that RNA transcripts are associated with DNA during all stages of mitosis. Reduction of H3K27me3 levels or elimination of RNAs had no effect on the association of the components of PcG and TrxG complexes to DNA. Using a combination of proximity ligation assay-based techniques and super-resolution microscopy, we found that the number of protein-DNA and RNA-DNA foci undergoes significant reduction during mitosis, suggesting that mitosis may be accompanied by structural re-arrangement or compaction of specific chromatin domains.
PubMed ID
PubMed Central ID
PMC5078801 (PMC) (EuropePMC)
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Cell Discov.
    Title
    Cell discovery
    ISBN/ISSN
    2056-5968
    Data From Reference