The RMCE-MiMIC Trojan-GAL4 collection consists of transgenic fly stocks in which the RMCE cassette in a progenitor Mi{MIC} insertion has been replaced with a gene trap cassette containing a Trojan GAL4 exon composed of a splice acceptor, T2A peptide, GAL4 coding sequence and an Hsp70 transcription termination signal. The RMCE donor constructs and methods described by Diao et al., 2015, Cell Rep. 10(8): 1410--1421 (FBrf0227693) were used for making the RMCE replacement. PCR is used to identify RMCE events that inserted the cassette in the correct orientation. The potential line is then crossed to UAS-2x-EGFP and its expression is evaluated with anti-GFP antibodies to identify gene trap lines where GAL4 is expressed under the control of the regulatory sequences of the trapped gene.
The 'Associated Files' for this personal communication consist of .xlsx files that were submitted to FlyBase at the time that each set of the fly stocks was sent to the Bloomington Drosophila Stock Center. The .xlsx files contain data primarily concerning the progenitor MiMIC line used, the phase of the donor construct used in the RMCE event, and the identities of any gene(s) in which the RMCE-MiMIC element is inserted. The gene associations are based on the FlyBase gene annotations at the time of submission of the data. The column "Trap? Y/N" for each gene indicates whether the gene trap cassette is predicted to result in the truncation of the trapped gene and expression of GAL4. Note that this is a prediction and does not necessarily indicate whether or not there is experimental evidence. For genes with multiple annotated transcript isoforms, a "Y" (Yes) in this column indicates that the insertion is predicted to truncate the protein translated from some, but not necessarily all, of the predictive transcript isoforms of the gene.  Similarly, GAL4 is predicted to be expressed only in cells expressing a transcript isoform in which the insertion acts as a gene trap (i.e. is inserted in an intron between two protein coding exons).