Open Close
Reference
Citation
Transgenic RNAi Project members, (2017-). Data submitted by the Transgenic RNAi Project to FlyBase for the TRiP-CRISPR Overexpression (TRiP-OE) and TRiP-CRISPR Knockout (TRiP-KO) transgenic construct collections. 
FlyBase ID
FBrf0234795
Publication Type
Personal communication to FlyBase
Abstract
PubMed ID
PubMed Central ID
Text of Personal Communication
The TRiP-CRISPR Overexpression (TRiP-OE) collection consists of fly stocks containing transgenic constructs designed for CRISPR/Cas9-based transcriptional activation of a gene of interest. 
	In the TRiP-OE-VPR collection, each construct constitutively expresses two sgRNAs that target ~500 base pairs upstream of the transcriptional start site of the targeted gene. When crossed to a stock that contains a source of nuclease-dead Cas9 fused to a transcriptional activator domain, such as dCas9-VPR, the gene of interest will be over-expressed in the domain of expression of this activator.
	In the TRiP-OE-flySAM collection, each construct constitutively expresses a single sgRNA that contains MS2 loops. The tagged sgRNA forms one component of the three-component flySAM (synergistic activation mediator) system, which is designed to produce effective transcriptional activation of the gene targeted by the sgRNA.
	In the TRiP-OE-flySAM.dCas9 collection, each construct constitutively expresses a single sgRNA that contains MS2 loops. The tagged sgRNA forms one component of the three-component flySAM (synergistic activation mediator) system, which is designed to produce effective transcriptional activation of the gene targeted by the sgRNA. In this collection, each construct also contains the other two components of the flySAM system (nuclease-dead Cas9 fused to a transcriptional activation domain plus an MCP-tagged transcriptional activator), and so target gene expression can be activated by crossing to any GAL4 line.
The TRiP-CRISPR Knockout (TRiP-KO) collection consists of fly stocks containing transgenic constructs designed for CRISPR/Cas9-based mutation of a gene of interest. Each construct constitutively expresses an sgRNA that targets the coding sequence of a gene.  When crossed to a stock that contains a source of Cas9, cleavage and mutagenesis of this target gene is induced, resulting in an indel (small insertion or deletion mutation).
The 'Associated Files' for this personal communication consist of .xlsx files that were submitted to FlyBase at the time that each set of fly stocks was sent to the Bloomington Drosophila Stock Center. The .xlsx files contain data concerning the vector used to generate the construct, its type (TRiP-OE or TRiP-KO) and the gene(s) targeted by the sgRNA(s).
DOI
Associated Information
Comments
Associated Files
File date: 2017.2.3 ; File size: 22334 ; File format: xlsx ; File name: gRNA_BDSC_20170203.xlsx
File date: 2017.10.23 ; File size: 29358 ; File format: xlsx ; File name: gRNA_BDSC_20171023.xlsx
File date: 2017.11.29 ; File size: 20564 ; File format: xlsx ; File name: gRNA_BDSC_20171129.xlsx
File date: 2018.9.24 ; File size: 38381 ; File format: xlsx ; File name: BDSC_20180924.xlsx
File date: 2018.10.22 ; File size: 53385 ; File format: xlsx ; File name: BDSC_sg_20181022.xlsx
File date: 2018.11.28 ; File size: 30619 ; File format: xlsx ; File name: gRNA_BDSC_2018-11-28.xlsx
File date: 2019.3.18 ; File size: 31182 ; File format: xlsx ; File name: bdsc_20190318.xlsx
File date: 2019.4.8 ; File size: 76566 ; File format: xlsx ; File name: BDSC_20190408.xlsx
File date: 2019.6.3 ; File size: 72352 ; File format: xlsx ; File name: BDSC_sgRNA_20190603.xlsx
File date: 2019.7.8 ; File size: 77506 ; File format: xlsx ; File name: BDSC_20190708.sgRNA.xlsx
File date: 2019.9.16 ; File size: 33775 ; File format: xlsx ; File name: BDSC_20190916.xlsx
File date: 2019.9.23 ; File size: 73021 ; File format: xlsx ; File name: BDSC_20190923.xlsx
File date: 2017.12.11 ; File size: 11589 ; File format: xlsx ; File name: gRNA_BDSC_20171211.xlsx
File date: 2017.5.4 ; File size: 29005 ; File format: xlsx ; File name: gRNA_BDSC_shipment_20170504.xlsx
File date: 2017.7.19 ; File size: 17163 ; File format: xlsx ; File name: gRNA_BDSC_shipment_20170710.xlsx
File date: 2018.3.19 ; File size: 36227 ; File format: xlsx ; File name: gRNA_BDSC_20180319.xlsx
File date: 2018.4.16 ; File size: 37980 ; File format: xlsx ; File name: BDSC_shipment9_final_file_20180407.xlsx
File date: 2018.8.13 ; File size: 37843 ; File format: xlsx ; File name: Sg_20180813.xlsx
File date: 2018.10.9 ; File size: 81320 ; File format: xlsx ; File name: BDSC_20181009.xlsx
File date: 2017.11.6 ; File size: 29400 ; File format: xlsx ; File name: gRNA_BDSC_20171106.xlsx
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Abbreviation
    Title
    ISBN/ISSN
    Data From Reference
    Alleles (3805)
    List limited to the first 200 records. Use the HitList export button in the left sidebar to view all records.
    Genes (2975)
    Datasets (5)
    Molecular Constructs (3)
    Sequence Features (5286)
    List limited to the first 200 records. Use the HitList export button in the left sidebar to view all records.
    Natural transposons (1)
    Insertions (3762)
    List limited to the first 200 records. Use the HitList export button in the left sidebar to view all records.
    Experimental Tools (1)
    Transgenic Constructs (3761)
    List limited to the first 200 records. Use the HitList export button in the left sidebar to view all records.