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Shan, L., Wu, C., Chen, D., Hou, L., Li, X., Wang, L., Chu, X., Hou, Y., Wang, Z. (2017). Regulators of alternative polyadenylation operate at the transition from mitosis to meiosis.  J. Genet. Genomics 44(2): 95--106.
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FBrf0234954
Publication Type
Research paper
Abstract

In the sexually reproductive organisms, gametes are produced by meiosis following a limited mitotic amplification. However, the intrinsic program switching cells from mitotic to meiotic cycle is unclear. Alternative polyadenylation (APA) is a highly conserved means of gene regulation and is achieved by the RNA 3'-processing machinery to generate diverse 3'UTR profiles. In Drosophila spermatogenesis, we observed distinct profiles of transcriptome-wide 3'UTR between mitotic and meiotic cells. In mutant germ cells stuck in mitosis, 3'UTRs of hundreds of genes were consistently shifted. Remarkably, altering the levels of multiple 3'-processing factors disrupted germline's progression to meiosis, indicative of APA's active role in this transition. An RNA-binding protein (RBP) Tut could directly bind 3'UTRs of 3'-processing factors whose expressions were repressed in the presence of Tut-containing complex. Further, we demonstrated that this RBP complex could execute the repression post-transcriptionally by recruiting CCR4/Twin of deadenylation complex. Thus, we propose that an RBP complex regulates the dynamic APA profile to promote the mitosis-to-meiosis transition.

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    J. Genet. Genomics
    Title
    Journal of Genetics and Genomics [Yi chuan xue bao]
    Publication Year
    2007--
    ISBN/ISSN
    1673-8527
    Data From Reference
    Alleles (77)
    Gene Groups (2)
    Genes (41)
    Physical Interactions (14)
    Cell Lines (1)
    Natural transposons (1)
    Insertions (11)
    Experimental Tools (4)
    Transgenic Constructs (65)