Open Close
Reference
Citation
Rogers, W.A., Goyal, Y., Yamaya, K., Shvartsman, S.Y., Levine, M.S. (2017). Uncoupling neurogenic gene networks in the Drosophila embryo.  Genes Dev. 31(7): 634--638.
FlyBase ID
FBrf0235399
Publication Type
Research paper
Abstract

The EGF signaling pathway specifies neuronal identities in the Drosophila embryo by regulating developmental patterning genes such as intermediate neuroblasts defective (ind). EGFR is activated in the ventral midline and neurogenic ectoderm by the Spitz ligand, which is processed by the Rhomboid protease. CRISPR/Cas9 was used to delete defined rhomboid enhancers mediating expression at each site of Spitz processing. Surprisingly, the neurogenic ectoderm, not the ventral midline, was found to be the dominant source of EGF patterning activity. We suggest that Drosophila is undergoing an evolutionary transition in central nervous system (CNS)-organizing activity from the ventral midline to the neurogenic ectoderm.

PubMed ID
PubMed Central ID
PMC5411704 (PMC) (EuropePMC)
Related Publication(s)
Note

Creating cell type-specific mutants by enhancer mutagenesis.
Crews, 2017, Genes Dev. 31(7): 629--631 [FBrf0235443]

Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Genes Dev.
    Title
    Genes & Development
    Publication Year
    1987-
    ISBN/ISSN
    0890-9369
    Data From Reference
    Alleles (10)
    Genes (6)
    Natural transposons (1)
    Insertions (7)
    Experimental Tools (2)
    Transgenic Constructs (2)