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Citation
Perez-Mockus, G., Roca, V., Mazouni, K., Schweisguth, F. (2017). Neuralized regulates Crumbs endocytosis and epithelium morphogenesis via specific Stardust isoforms.  J. Cell Biol. 216(5): 1405--1420.
FlyBase ID
FBrf0235454
Publication Type
Research paper
Abstract
Crumbs (Crb) is a conserved determinant of apical membrane identity that regulates epithelial morphogenesis in many developmental contexts. In this study, we identify the Crb complex protein Stardust (Sdt) as a target of the E3 ubiquitin ligase Neuralized (Neur) in Drosophila melanogaster Neur interacts with and down-regulates specific Sdt isoforms containing a Neur binding motif (NBM). Using a CRISPR (clustered regularly interspaced short palindromic repeats)-induced deletion of the NBM-encoding exon, we found that Sdt is a key Neur target and that Neur acts via Sdt to down-regulate Crb. We further show that Neur promotes the endocytosis of Crb via the NBM-containing isoforms of Sdt. Although the regulation of Crb by Neur is not strictly essential, it contributes to epithelium remodeling in the posterior midgut and thereby facilitates the trans-epithelial migration of the primordial germ cells in early embryos. Thus, our study uncovers a novel regulatory mechanism for the developmental control of Crb-mediated morphogenesis.
PubMed ID
PubMed Central ID
PMC5412571 (PMC) (EuropePMC)
Related Publication(s)
Note
Stardust, the Janus-faced partner of Crumbs.
Das and Knust, 2017, J. Cell Biol. 216(5): 1219--1221 [FBrf0235511]
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    J. Cell Biol.
    Title
    Journal of Cell Biology
    Publication Year
    1966-
    ISBN/ISSN
    0021-9525
    Data From Reference