Open Close
Reference
Citation
Vanhove, A.S., Hang, S., Vijayakumar, V., Wong, A.C., Asara, J.M., Watnick, P.I. (2017). Vibrio cholerae ensures function of host proteins required for virulence through consumption of luminal methionine sulfoxide.  PLoS Pathog. 13(6): e1006428.
FlyBase ID
FBrf0235834
Publication Type
Research paper
Abstract

Vibrio cholerae is a diarrheal pathogen that induces accumulation of lipid droplets in enterocytes, leading to lethal infection of the model host Drosophila melanogaster. Through untargeted lipidomics, we provide evidence that this process is the product of a host phospholipid degradation cascade that induces lipid droplet coalescence in enterocytes. This infection-induced cascade is inhibited by mutation of the V. cholerae glycine cleavage system due to intestinal accumulation of methionine sulfoxide (MetO), and both dietary supplementation with MetO and enterocyte knock-down of host methionine sulfoxide reductase A (MsrA) yield increased resistance to infection. MsrA converts both free and protein-associated MetO to methionine. These findings support a model in which dietary MetO competitively inhibits repair of host proteins by MsrA. Bacterial virulence strategies depend on functional host proteins. We propose a novel virulence paradigm in which an intestinal pathogen ensures the repair of host proteins essential for pathogenesis through consumption of dietary MetO.

PubMed ID
PubMed Central ID
PMC5473594 (PMC) (EuropePMC)
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    PLoS Pathog.
    Title
    PLoS Pathogens
    Publication Year
    2005-
    ISBN/ISSN
    1553-7366 1553-7374
    Data From Reference
    Alleles (6)
    Genes (3)
    Human Disease Models (1)
    Insertions (1)
    Transgenic Constructs (3)