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Pae, J., Cinalli, R.M., Marzio, A., Pagano, M., Lehmann, R. (2017). GCL and CUL3 Control the Switch between Cell Lineages by Mediating Localized Degradation of an RTK.  Dev. Cell 42(2): 130--142.e7.
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Research paper

The separation of germline from somatic lineages is fundamental to reproduction and species preservation. Here, we show that Drosophila Germ cell-less (GCL) is a critical component in this process by acting as a switch that turns off a somatic lineage pathway. GCL, a conserved BTB (Broad-complex, Tramtrack, and Bric-a-brac) protein, is a substrate-specific adaptor for Cullin3-RING ubiquitin ligase complex (CRL3(GCL)). We show that CRL3(GCL) promotes PGC fate by mediating degradation of Torso, a receptor tyrosine kinase (RTK) and major determinant of somatic cell fate. This mode of RTK degradation does not depend upon receptor activation but is prompted by release of GCL from the nuclear envelope during mitosis. The cell-cycle-dependent change in GCL localization provides spatiotemporal specificity for RTK degradation and sequesters CRL3(GCL) to prevent it from participating in excessive activities. This precisely orchestrated mechanism of CRL3(GCL) function and regulation defines cell fate at the single-cell level.

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Obtained with permission from Cell Press.
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PubMed Central ID
PMC5568677 (PMC) (EuropePMC)
Related Publication(s)

How Primordial Germ Cells Destroy Somatic Signals.
Cooley, 2017, Dev. Cell 42(2): 107--108 [FBrf0236189]

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    Language of Publication
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    Parent Publication
    Publication Type
    Dev. Cell
    Developmental Cell
    Publication Year
    1534-5807 1878-1551
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