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Citation
Song, Y., Marmion, R.A., Park, J.O., Biswas, D., Rabinowitz, J.D., Shvartsman, S.Y. (2017). Dynamic Control of dNTP Synthesis in Early Embryos.  Dev. Cell 42(3): 301--308.e3.
FlyBase ID
FBrf0236236
Publication Type
Research paper
Abstract

Exponential increase of cell numbers in early embryos requires large amounts of DNA precursors (deoxyribonucleoside triphosphates (dNTPs)). Little is understood about how embryos satisfy this demand. We examined dNTP metabolism in the early Drosophila embryo, in which gastrulation is preceded by 13 sequential nuclear cleavages within only 2 hr of fertilization. Surprisingly, despite the breakneck speed at which Drosophila embryos synthesize DNA, maternally deposited dNTPs can generate less than half of the genomes needed to reach gastrulation. The rest of the dNTPs are synthesized "on the go." The rate-limiting enzyme of dNTP synthesis, ribonucleotide reductase, is inhibited by endogenous levels of deoxyATP (dATP) present at fertilization and is activated as dATP is depleted via DNA polymerization. This feedback inhibition renders the concentration of dNTPs at gastrulation robust, with respect to large variations in maternal supplies, and is essential for normal progression of embryogenesis.

PubMed ID
PubMed Central ID
PMC5630127 (PMC) (EuropePMC)
Related Publication(s)
Note

For Embryos, Mother Can Only Take You So Far.
Ferree and Di Talia, 2017, Dev. Cell 42(3): 203--205 [FBrf0236334]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Dev. Cell
    Title
    Developmental Cell
    Publication Year
    2001-
    ISBN/ISSN
    1534-5807 1878-1551
    Data From Reference
    Alleles (6)
    Genes (3)
    Natural transposons (1)
    Experimental Tools (1)
    Transgenic Constructs (5)