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Citation
Fanti, L., Piacentini, L., Cappucci, U., Casale, A.M., Pimpinelli, S. (2017). Canalization by Selection of de Novo Induced Mutations.  Genetics 206(4): 1995--2006.
FlyBase ID
FBrf0236262
Publication Type
Research paper
Abstract
One of the most fascinating scientific problems, and a subject of intense debate, is that of the mechanisms of biological evolution. In this context, Waddington elaborated the concepts of "canalization and assimilation" to explain how an apparently somatic variant induced by stress could become heritable through the germline in Drosophila He resolved this seemingly Lamarckian phenomenon by positing the existence of cryptic mutations that can be expressed and selected under stress. To investigate the relevance of such mechanisms, we performed experiments following the Waddington procedure, then isolated and fixed three phenotypic variants along with another induced mutation that was not preceded by any phenocopy. All the fixed mutations we looked at were actually generated de novo by DNA deletions or transposon insertions, highlighting a novel mechanism for the assimilation process. Our study shows that heat-shock stress produces both phenotypic variants and germline mutations, and suggests an alternative explanation to that of Waddington for the apparent assimilation of an acquired character. The selection of the variants, under stress, for a number of generations allows for the coselection of newly induced corresponding germline mutations, making the phenotypic variants appear heritable.
PubMed ID
PubMed Central ID
PMC5560803 (PMC) (EuropePMC)
Related Publication(s)
Note
Waddington Redux: De Novo Mutations Underlie the Genetic Assimilation of Stress-Induced Phenocopies in Drosophila melanogaster.
Kasinathan et al., 2017, Genetics 207(1): 49--51 [FBrf0236584]
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Genetics
    Title
    Genetics
    Publication Year
    1916-
    ISBN/ISSN
    0016-6731
    Data From Reference
    Aberrations (1)
    Alleles (8)
    Genes (5)
    Natural transposons (7)
    Insertions (2)
    Transgenic Constructs (1)