Li, H., Watson, A., Olechwier, A., Anaya, M., Sorooshyari, S.K., Harnett, D.P., Lee, H.P., Vielmetter, J., Fares, M.A., Garcia, K.C., Özkan, E., Labrador, J.P., Zinn, K. (2017). Deconstruction of the beaten Path-Sidestep interaction network provides insights into neuromuscular system development.  eLife 6(): e28111.

FBrf0236547

Research paper

An 'interactome' screen of all Drosophila cell-surface and secreted proteins containing immunoglobulin superfamily (IgSF) domains discovered a network formed by paralogs of Beaten Path (Beat) and Sidestep (Side), a ligand-receptor pair that is central to motor axon guidance. Here we describe a new method for interactome screening, the Bio-Plex Interactome Assay (BPIA), which allows identification of many interactions in a single sample. Using the BPIA, we 'deorphanized' four more members of the Beat-Side network. We confirmed interactions using surface plasmon resonance. The expression patterns of beat and side genes suggest that Beats are neuronal receptors for Sides expressed on peripheral tissues. side-VI is expressed in muscle fibers targeted by the ISNb nerve, as well as at growth cone choice points and synaptic targets for the ISN and TN nerves. beat-V genes, encoding Side-VI receptors, are expressed in ISNb and ISN motor neurons.

PMC5578738 (PMC) (EuropePMC)