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Citation
Church, V.A., Pressman, S., Isaji, M., Truscott, M., Cizmecioglu, N.T., Buratowski, S., Frolov, M.V., Carthew, R.W. (2017). Microprocessor Recruitment to Elongating RNA Polymerase II Is Required for Differential Expression of MicroRNAs.  Cell Rep. 20(13): 3123--3134.
FlyBase ID
FBrf0236708
Publication Type
Research paper
Abstract

The cellular abundance of mature microRNAs (miRNAs) is dictated by the efficiency of nuclear processing of primary miRNA transcripts (pri-miRNAs) into pre-miRNA intermediates. The Microprocessor complex of Drosha and DGCR8 carries this out, but it has been unclear what controls Microprocessor's differential processing of various pri-miRNAs. Here, we show that Drosophila DGCR8 (Pasha) directly associates with the C-terminal domain of the RNA polymerase II elongation complex when it is phosphorylated by the Cdk9 kinase (pTEFb). When association is blocked by loss of Cdk9 activity, a global change in pri-miRNA processing is detected. Processing of pri-miRNAs with a UGU sequence motif in their apical junction domain increases, while processing of pri-miRNAs lacking this motif decreases. Therefore, phosphorylation of RNA polymerase II recruits Microprocessor for co-transcriptional processing of non-UGU pri-miRNAs that would otherwise be poorly processed. In contrast, UGU-positive pri-miRNAs are robustly processed by Microprocessor independent of RNA polymerase association.

Graphical Abstract
Obtained with permission from Cell Press.
PubMed ID
PubMed Central ID
PMC5639929 (PMC) (EuropePMC)
Related Publication(s)
Personal communication to FlyBase

Location data for Cdk9[+t2.5] rescue allele.
Carthew, 18.1.30, Location data for Cdk9[+t2.5] rescue allele. [FBrf0237929]

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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Cell Rep.
    Title
    Cell reports
    ISBN/ISSN
    2211-1247
    Data From Reference
    Aberrations (2)
    Alleles (5)
    Genes (18)
    Physical Interactions (4)
    Cell Lines (1)
    Natural transposons (1)
    Transgenic Constructs (2)