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Citation
Riedel, F., Galindo, A., Muschalik, N., Munro, S. (2018). The two TRAPP complexes of metazoans have distinct roles and act on different Rab GTPases.  J. Cell Biol. 217(2): 601--617.
FlyBase ID
FBrf0238107
Publication Type
Research paper
Abstract

Originally identified in yeast, transport protein particle (TRAPP) complexes are Rab GTPase exchange factors that share a core set of subunits. TRAPPs were initially found to act on Ypt1, the yeast orthologue of Rab1, but recent studies have found that yeast TRAPPII can also activate the Rab11 orthologues Ypt31/32. Mammals have two TRAPP complexes, but their role is less clear, and they contain subunits that are not found in the yeast complexes but are essential for cell growth. To investigate TRAPP function in metazoans, we show that Drosophila melanogaster have two TRAPP complexes similar to those in mammals and that both activate Rab1, whereas one, TRAPPII, also activates Rab11. TRAPPII is not essential but becomes so in the absence of the gene parcas that encodes the Drosophila orthologue of the SH3BP5 family of Rab11 guanine nucleotide exchange factors (GEFs). Thus, in metazoans, Rab1 activation requires TRAPP subunits not found in yeast, and Rab11 activation is shared by TRAPPII and an unrelated GEF that is metazoan specific.

PubMed ID
PubMed Central ID
PMC5800803 (PMC) (EuropePMC)
Related Publication(s)
Personal communication to FlyBase

Location data for brun[105] deletion.
Riedel and Munro, 2018.8.13, Location data for brun[105] deletion. [FBrf0239827]

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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    J. Cell Biol.
    Title
    Journal of Cell Biology
    Publication Year
    1966-
    ISBN/ISSN
    0021-9525
    Data From Reference
    Aberrations (5)
    Alleles (58)
    Gene Groups (2)
    Genes (36)
    Physical Interactions (77)
    Cell Lines (1)
    Insertions (5)
    Experimental Tools (3)
    Transgenic Constructs (1)