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Citation
Lee, P.T., Zirin, J., Kanca, O., Lin, W.W., Schulze, K.L., Li-Kroeger, D., Tao, R., Devereaux, C., Hu, Y., Chung, V., Fang, Y., He, Y., Pan, H., Ge, M., Zuo, Z., Housden, B.E., Mohr, S.E., Yamamoto, S., Levis, R.W., Spradling, A.C., Perrimon, N., Bellen, H.J. (2018). A gene-specific T2A-GAL4 library for Drosophila.  eLife 7(): e35574.
FlyBase ID
FBrf0238661
Publication Type
Research paper
Abstract
We generated a library of ~1000 Drosophila stocks in which we inserted a construct in the intron of genes allowing expression of GAL4 under control of endogenous promoters while arresting transcription with a polyadenylation signal 3' of the GAL4. This allows numerous applications. First, ~90% of insertions in essential genes cause a severe loss-of-function phenotype, an effective way to mutagenize genes. Interestingly, 12/14 chromosomes engineered through CRISPR do not carry second-site lethal mutations. Second, 26/36 (70%) of lethal insertions tested are rescued with a single UAS-cDNA construct. Third, loss-of-function phenotypes associated with many GAL4 insertions can be reverted by excision with UAS-flippase. Fourth, GAL4 driven UAS-GFP/RFP reports tissue and cell-type specificity of gene expression with high sensitivity. We report the expression of hundreds of genes not previously reported. Finally, inserted cassettes can be replaced with GFP or any DNA. These stocks comprise a powerful resource for assessing gene function.
PubMed ID
PubMed Central ID
PMC5898912 (PMC) (EuropePMC)
Related Publication(s)
Personal communication to FlyBase
Data submitted by the Gene Disruption Project to FlyBase for the CRIMIC insertion line collection.
Gene Disruption Project members, 2018-, Data submitted by the Gene Disruption Project to FlyBase for the CRIMIC insertion line collection. [FBrf0239542]
Additional information about CRIMIC insertion lines described in FBrf0238661.
Levis, 2018.8.30, Additional information about CRIMIC insertion lines described in FBrf0238661. [FBrf0240219]
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    eLife
    Title
    eLife
    ISBN/ISSN
    2050-084X
    Data From Reference
    Aberrations (106)
    Alleles (320)
    List limited to the first 200 records. Use the HitList export button in the left sidebar to view all records.
    Genes (221)
    Datasets (2)
    Molecular Constructs (5)
    Natural transposons (1)
    Insertions (1152)
    List limited to the first 200 records. Use the HitList export button in the left sidebar to view all records.
    Experimental Tools (6)
    Transgenic Constructs (46)