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Citation
Shearin, H.K., Quinn, C.D., Mackin, R.D., Macdonald, I.S., Stowers, R.S. (2018). t-GRASP, a targeted GRASP for assessing neuronal connectivity.  J. Neurosci. Methods 306(): 94--102.
FlyBase ID
FBrf0239257
Publication Type
Research paper
Abstract

Understanding how behaviors are generated by neural circuits requires knowledge of the synaptic connections between the composite neurons. Methods for mapping synaptic connections, such as electron microscopy and paired recordings, are labor intensive and alternative methods are thus desirable. Development of a targeted GFP Reconstitution Across Synaptic Partners(GRASP) method, t-GRASP, for assessing neural connectivity is described. Numerous different pre-synaptic and post-synaptic/dendritic proteins were tested for enhancing the specificity of GRASP signal to synaptic regions. Pairing of both targeted pre- and post-t-GRASP constructs resulted in strong preferential GRASP signal in synaptic regions in Drosophila larval sensory neurons, larval neuromuscular junctions, and adult photoreceptor neurons with minimal false-positive signal. Activity-independent t-GRASP exhibits an enhancement of GRASP signal specificity for synaptic contact sites as compared to existing Drosophila GRASP methods. Fly strains were developed for expression of both pre- and post-t-GRASP with each of the three Drosophila binary transcription systems, thus enabling GRASP assays to be performed between any two driver pairs of any transcription system in either direction, an option not available for existing Drosophila GRASP methods. t-GRASP is a novel targeted GRASP method for assessing synaptic connectivity between Drosophila neurons. Its flexibility of use with all three Drosophila binary transcription systems significantly expands the potential use of GRASP in Drosophila.

PubMed ID
PubMed Central ID
PMC6689385 (PMC) (EuropePMC)
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    J. Neurosci. Methods
    Title
    Journal of Neuroscience Methods
    Publication Year
    1979-
    ISBN/ISSN
    0165-0270
    Data From Reference
    Alleles (50)
    Genes (17)
    Natural transposons (2)
    Insertions (26)
    Experimental Tools (11)
    Transgenic Constructs (48)