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Reference
Citation
Sapar, M.L., Ji, H., Wang, B., Poe, A.R., Dubey, K., Ren, X., Ni, J.Q., Han, C. (2018). Phosphatidylserine Externalization Results from and Causes Neurite Degeneration in Drosophila.  Cell Rep. 24(9): 2273--2286.
FlyBase ID
FBrf0239914
Publication Type
Research paper
Abstract

Phagocytic clearance of degenerating dendrites or axons is critical for maintaining tissue homeostasis and preventing neuroinflammation. Externalized phosphatidylserine (PS) has been postulated to be an "eat-me" signal allowing recognition of degenerating neurites by phagocytes. Here we show that in Drosophila, PS is dynamically exposed on degenerating dendrites during developmental pruning and after physical injury, but PS exposure is suppressed when dendrite degeneration is genetically blocked. Ectopic PS exposure via phospholipid flippase knockout and scramblase overexpression induced PS exposure preferentially at distal dendrites and caused distinct modes of neurite loss that differ in larval sensory dendrites and in adult olfactory axons. Surprisingly, extracellular lactadherin that lacks the integrin-interaction domain induced phagocyte-dependent degeneration of PS-exposing dendrites, revealing an unidentified bridging function that potentiates phagocytes. Our findings establish a direct causal relationship between PS exposure and neurite degeneration in vivo.

PubMed ID
PubMed Central ID
PMC6174084 (PMC) (EuropePMC)
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Cell Rep.
    Title
    Cell reports
    ISBN/ISSN
    2211-1247
    Data From Reference
    Alleles (29)
    Genes (19)
    Sequence Features (1)
    Natural transposons (2)
    Insertions (5)
    Experimental Tools (11)
    Transgenic Constructs (26)
    Transcripts (1)