Open Close
Reference
Citation
Yao, B., Li, Y., Wang, Z., Chen, L., Poidevin, M., Zhang, C., Lin, L., Wang, F., Bao, H., Jiao, B., Lim, J., Cheng, Y., Huang, L., Phillips, B.L., Xu, T., Duan, R., Moberg, K.H., Wu, H., Jin, P. (2018). Active N6-Methyladenine Demethylation by DMAD Regulates Gene Expression by Coordinating with Polycomb Protein in Neurons.  Mol. Cell 71(5): 848--857.e6.
FlyBase ID
FBrf0240003
Publication Type
Research paper
Abstract

A ten-eleven translocation (TET) ortholog exists as a DNA N6-methyladenine (6mA) demethylase (DMAD) in Drosophila. However, the molecular roles of 6mA and DMAD remain unexplored. Through genome-wide 6mA and transcriptome profiling in Drosophila brains and neuronal cells, we found that 6mA may epigenetically regulate a group of genes involved in neurodevelopment and neuronal functions. Mechanistically, DMAD interacts with the Trithorax-related complex protein Wds to maintain active transcription by dynamically demethylating intragenic 6mA. Accumulation of 6mA by depleting DMAD coordinates with Polycomb proteins and contributes to transcriptional repression of these genes. Our findings suggest that active 6mA demethylation by DMAD plays essential roles in fly CNS by orchestrating through added epigenetic mechanisms.

PubMed ID
PubMed Central ID
PMC6136845 (PMC) (EuropePMC)
Associated Information
Comments
Associated Files
Other Information
Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Mol. Cell
    Title
    Molecular Cell
    Publication Year
    1997-
    ISBN/ISSN
    1097-2765 1097-4164
    Data From Reference
    Genes (3)
    Physical Interactions (4)
    Cell Lines (1)