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Citation
Kistler, K.E., Trcek, T., Hurd, T.R., Chen, R., Liang, F.X., Sall, J., Kato, M., Lehmann, R. (2018). Phase transitioned nuclear Oskar promotes cell division of Drosophila primordial germ cells.  eLife 7(): e37949.
FlyBase ID
FBrf0240356
Publication Type
Research paper
Abstract

Germ granules are non-membranous ribonucleoprotein granules deemed the hubs for post-transcriptional gene regulation and functionally linked to germ cell fate across species. Little is known about the physical properties of germ granules and how these relate to germ cell function. Here we study two types of germ granules in the Drosophila embryo: cytoplasmic germ granules that instruct primordial germ cells (PGCs) formation and nuclear germ granules within early PGCs with unknown function. We show that cytoplasmic and nuclear germ granules are phase transitioned condensates nucleated by Oskar protein that display liquid as well as hydrogel-like properties. Focusing on nuclear granules, we find that Oskar drives their formation in heterologous cell systems. Multiple, independent Oskar protein domains synergize to promote granule phase separation. Deletion of Oskar's nuclear localization sequence specifically ablates nuclear granules in cell systems. In the embryo, nuclear germ granules promote germ cell divisions thereby increasing PGC number for the next generation.

PubMed ID
PubMed Central ID
PMC6191285 (PMC) (EuropePMC)
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    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    eLife
    Title
    eLife
    ISBN/ISSN
    2050-084X
    Data From Reference
    Aberrations (1)
    Alleles (6)
    Genes (7)
    Cell Lines (1)
    Insertions (1)
    Transgenic Constructs (2)