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Citation
Panda, A., Thakur, R., Krishnan, H., Naik, A., Shinde, D., Raghu, P. (2018). Functional analysis of mammalian phospholipase D enzymes.  Biosci. Rep. 38(6): BSR20181690.
FlyBase ID
FBrf0240909
Publication Type
Research paper
Abstract
Phosphatidylcholine (PC)-specific phospholipase D (PLD) hydrolyzes the phosphodiester bond of the PC to generate phosphatidic acid (PA) and regulates several subcellular functions. Mammalian genomes contain two genes encoding distinct isoforms of PLD in contrast with invertebrate genomes that include a single PLD gene. However, the significance of two genes within a genome encoding the same biochemical activity remains unclear. Recently, loss of function in the only PLD gene in Drosophila was reported to result in reduced PA levels and a PA-dependent collapse of the photoreceptor plasma membrane due to defects in vesicular transport. Phylogenetic analysis reveals that human PLD1 (hPLD1) is evolutionarily closer to dPLD than human PLD2 (hPLD2). In the present study, we expressed hPLD1 and hPLD2 in Drosophila and found that while reconstitution of hPLD1 is able to completely rescue retinal degeneration in a loss of function dPLD mutant, hPLD2 was less effective in its ability to mediate a rescue. Using a newly developed analytical method, we determined the acyl chain composition of PA species produced by each enzyme. While dPLD was able to restore the levels of most PA species in dPLD3.1 cells, hPLD1 and hPLD2 each were unable to restore the levels of a subset of unique species of PA. Finally, we found that in contrast with hPLD2, dPLD and hPLD1 are uniquely distributed to the subplasma membrane region in photoreceptors. In summary, hPLD1 likely represents the ancestral PLD in mammalian genomes while hPLD2 represents neofunctionalization to generate PA at distinct subcellular membranes.
PubMed ID
PubMed Central ID
PMC6435507 (PMC) (EuropePMC)
Related Publication(s)
Note
Phospholipases D: making sense of redundancy and duplication.
Morris, 2019, Biosci. Rep. 39(6): BSR20181883 [FBrf0242766]
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Secondary IDs
    Language of Publication
    English
    Additional Languages of Abstract
    Parent Publication
    Publication Type
    Journal
    Abbreviation
    Biosci. Rep.
    Title
    Bioscience Reports
    Publication Year
    1981-
    ISBN/ISSN
    0144-8463
    Data From Reference
    Alleles (9)
    Genes (5)
    Cell Lines (1)
    Natural transposons (1)
    Experimental Tools (3)
    Transgenic Constructs (7)