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Wu, D., Wu, L., An, H., Bao, H., Guo, P., Zhang, B., Zheng, H., Zhang, F., Ge, W., Cai, Y., Xi, Y., Yang, X. (2019). RanGAP-mediated nucleocytoplasmic transport of Prospero regulates neural stem cell lifespan in Drosophila larval central brain.  Aging Cell 18(1): e12854.
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Research paper

By the end of neurogenesis in Drosophila pupal brain neuroblasts (NBs), nuclear Prospero (Pros) triggers cell cycle exit and terminates NB lifespan. Here, we reveal that in larval brain NBs, an intrinsic mechanism facilitates import and export of Pros across the nuclear envelope via a Ran-mediated nucleocytoplasmic transport system. In rangap mutants, the export of Pros from the nucleus to cytoplasm is impaired and the nucleocytoplasmic transport of Pros becomes one-way traffic, causing an early accumulation of Pros in the nuclei of the larval central brain NBs. This nuclear Pros retention initiates NB cell cycle exit and leads to a premature decrease of total NB numbers. Our data indicate that RanGAP plays a crucial role in this intrinsic mechanism that controls NB lifespan during neurogenesis. Our study may provide insights into understanding the lifespan of neural stem cells during neurogenesis in other organisms.

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PubMed Central ID
PMC6351831 (PMC) (EuropePMC)
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Personal communication to FlyBase

Location data for RanGAP mutations.
Litao et al., 2019.5.24, Location data for RanGAP mutations. [FBrf0244950]

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    Parent Publication
    Publication Type
    Aging Cell
    Aging Cell
    Publication Year
    1474-9718 1474-9728
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